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In the 1 week, the new lung area was sweaty with cooler PBS to get rid of bloodstream and you will thumb frozen in the h2o nitrogen. 5-15 milligrams from flash-suspended lung tissues try incubated with 0.2 mM CMH in the KHB containing one hundred μM inside the 200 μL of overall volume for example h from the 37° C. Supernatant are obtained and you will listed in PTFE tubing and you may work on in the 77 K from the digit dewar. (A) Nitroxide concentration (μM out of nitroxide normalized to at least one milligrams from tissue). (B) Associate spectra of nitroxide inside the lung tissues from PBS- and you will Bleo-managed rats. (A) Raw 264.7 tissues stimulated with ten μM PMA and EPR twist probe, CMH 0.25 mM (fifty min in the 37 °C) with (black) or instead of (red) pretreatment with 30 You/mL SOD.

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